Research at ³ÉÈË´óƬA technology for preventing probe clogging during mass spectrometry analysis of complex surfaces
Background
Mass spectrometry (MS) is a powerful analytical tool used to identify and quantify compounds in various samples. Traditional techniques like desorption electrospray ionization (DESI) and matrix-assisted laser desorption ionization (MALDI) have limitations, such as the need for uniform surfaces or extensive sample preparation. These methods often struggle with uneven or wet surfaces, presenting a significant challenge in fields like biological tissue analysis and microbial studies.
Technology Overview
This technology enables the analysis of problematic complex surfaces by detecting when the probe is sufficiently close to the sample, while preventing direct contact and clogging – a common issue in traditional approaches. This ensures accurate analyte desorption and efficient delivery to the mass spectrometer. Used with an automated probe positioner similar to a 3D printer, the technology incorporates a conductance feedback mechanism which halts the probe’s movement just before it touches the sample surface. This approach offers consistent, reproducible sampling and enables the automated analysis of multiple spots, providing spatially resolved data. The method has been successfully demonstrated in mapping variable concentrations of heme in beef tissue samples.
Benefits
- Direct Imaging: Enables analysis of uneven, wet, or dry samples without extensive preparation.
- High Throughput: Automates sampling across multiple spots, enhancing efficiency and reproducibility.
- Reduced Contamination: Continuous solvent flow minimizes contamination risks.
- Versatility: Suitable for various sample types, including biological tissues and microbial colonies.
- Enhanced Resolution: Potential for improved resolution by finely controlling the probe’s proximity to the sample.
Applications
- Mass Spectrometry Manufacturers: Companies like Sciex and Waters can incorporate this technology to enhance imaging capabilities.
- Analytical Science Laboratories: Labs can benefit from efficient and direct imaging of complex samples.
- Biological and Medical Research: Facilitates analysis of tissue samples and microbial studies.
Opportunity
Mass spectrometer manufacturers and analytical labs can gain significantly from integrating this technology. Current challenges include optimizing the conductance feedback system and developing integrated software for additional probe control. Collaboration with mass spectrometry companies can address these issues and further optimize the technology for diverse applications. Interested parties are invited to explore licensing and partnership opportunities to bring the technology to market.
Patents
- WO2022256941A1
- US20240274426A1
- CA3221826A1
IP Status
Patent application submitted
Seeking
- Development partner
- Commercial partner
- Licensing
Posted
September 25, 2024